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February 21, 2026Biophysical Journal

BPS2026 – Real-time imaging of rotation during synthesis by the replisome

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Authors

TRThomas M RetzerLRLional RajappaMTMasateru Takahashi

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Overview

Observations reveal replisome rotation impacts DNA synthesis and mechanical consequences in replication dynamics.

Key Points

  • This research aims to understand the mechanical dynamics of the replisome during DNA replication.
  • Developed a transverse DNA flow-stretching approach to observe replication dynamics
  • Used bacteriophage T7 as a model system for real-time imaging
  • Resolved the parental leading and lagging strands during synthesis.
  • Revealed bursts of high-speed replisome rotation supporting continuous DNA synthesis
  • Excessive rotation increases pausing and reduces processivity
  • Increased the number of polymerases during replication despite maintaining replisome speed.

Cite This Study

Retzer et al. (2026) studied this question.

synapsesocial.com/papers/69990de85b97ab4c14ac288chttps://doi.org/10.1016/j.bpj.2025.11.1209
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