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February 21, 2026Biophysical Journal

BPS2026 – Assembling large numbers of size-controlled giant unilamellar vesicles for biophysical applications

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Authors

VVVignesha VijayanandaASAnand Bala Subramaniam

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Overview

This technique produces billions of giant unilamellar vesicles, indicating potential for improved microfluidic experiments.

Key Points

  • To develop a method for producing large quantities of size-controlled giant unilamellar vesicles (GUVs) for biophysical applications.
  • Utilized a novel workflow called PAPYRUS for GUV assembly.
  • Produced over 10^9 GUVs in 70 mL of buffer within 2.5 hours.
  • Incorporated centrifugation and solvent exchange to concentrate GUVs for experiments.
  • Achieved high concentrations of GUVs, 300,000 per microliter in physiological saline.
  • Made GUVs predominantly under 10 μm, suitable for biological applications.
  • Facilitated enough output for 11 in vitro microfluidic experiments or 70 in vivo experiments within 3 hours.

Cite This Study

Vijayananda et al. (2026) studied this question.

synapsesocial.com/papers/69990de85b97ab4c14ac29afhttps://doi.org/10.1016/j.bpj.2025.11.2153
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Also Consider

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  1. 1Ultrahigh yields of giant vesicles obtained through mesophase evolution and breakup2024 · 1 citations
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  3. 3Mechanisms and Factors Influencing the Production of Uniform-Sized Giant Unilamellar Vesicles by Discrete Lipid Film Arrays2024
  4. 4Electroformation of giant unilamellar vesicles from large liposomes2024 · 3 citations
  5. 5Microfluidic production, stability and loading of synthetic giant unilamellar vesicles2024 · 9 citations