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February 21, 2026Biophysical Journal0 citations

Cold Activation of TRPM8 via High-Throughput Automated Patch Clamp Techniques

BPS2026 – Cold activation of TRPM8 using high-throughput automated patch clamp

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Authors

MRMaria Giustina RotordamJRJan RathjeNBNadine Becker

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Overview

Automated patch clamp methods activated TRPM8 in CHO cells, suggesting cold stimuli may aid drug discovery for neuropathic pain.

Key Points

  • To explore effective methods for activating TRPM8 using cold stimuli for drug discovery applications.
  • Utilized automated patch clamp systems for TRPM8 activation
  • Measured currents at varying temperatures (10-25°C)
  • Employed menthol and cold solutions for activation
  • Tested modulators such as capsazepine during experiments
  • TRPM8 activation led to increasing current density from 20 pA/pF at 25°C to 120 pA/pF at 10°C
  • Cold solution application successfully elicited TRPM8-mediated currents
  • Capsazepine blocked TRPM8 currents, confirming its modulatory role

Cite This Study

Rotordam et al. (2026) studied this question.

synapsesocial.com/papers/69990e015b97ab4c14ac2f61https://doi.org/10.1016/j.bpj.2025.11.1983
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