Mustn1 ablation in male mice results in fiber type and gene expression alterations during skeletal muscle regeneration

Figures

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  FIGURE 1. Histological analyses of tibialis anterior muscle. Representative images of WT (a–f) and KO (g–l) tibialis anterior muscle stained with H & E. Control (uninjured) and 0, 2, 5, and 10 days post‐injury. f and l are magnified regions depicted in e and k, respectively. Arrows indicate fibers with centrally located nuclei. Scale bar = 100 μm and 50 μm.

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  FIGURE 2. Muscle cross sectional area and central nucleated fibers. Muscle cross sectional area comparison between WT and Mustn1 KO mice for Type I (a), Type IIa (b) and Type Iib (c) fiber. (d) Centrally nucleated fibers WT and Mustn1 KO mice. Comparisons were made using a mixed‐effects model followed post‐hoc Tukey's tests to identify pairwise differences between genotypes and times.

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  FIGURE 3. Fiber typing: Quantitative data. (a) Type I fibers; (b) Type IIa fibers; (c) Type IIb fibers; (d) Type IIx fibers. Control refers to uninjured and Day 0–10 refer to post‐injured samples. All p‐values were calculated using a Tukey's multiple comparisons test.

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  FIGURE 4. Fiber typing: Qualitative data. Representative images show the various fiber types from each strain and time point. Control refers to uninjured and day 0–10 refer to post‐injured samples. For the Type Iix fiber, the images appear at a higher magnification so that each distinct fiber is shown: Type I (pink), Type IIa (red), Type IIb (green), and Type Iix (black). Scale bars = 250 and 50 μm.

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  FIGURE 5. Pax 7 expression. (a) Representative images show Pax7+ fibers from each strain and time point. Control refers to uninjured and Day 0–10 refer to post‐injured samples. (b) Graph indicates the quantitative data All p‐values were calculated using a Tukey's multiple comparisons test. Scale bar = 50 μm.

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  FIGURE 6. Gene expression analyses. Expression levels of myogenic marker genes (a) MyoD, (b) Myh4, (c) Cap1, (d) Des, (e) Cadh, and (f) Mustn1. Control refers to uninjured and Day 0–10 refer to post‐injured samples. All p‐values were calculated using a Tukey's multiple comparisons test.

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  FIGURE 7. Ladder climbing. (a) Ladder climbing comparison between WT and Mustn1 KO mice (n = 5/strain); (b) WT mice ladder climbing; (c) Mustn1 KO mice ladder climbing. All p‐values were calculated using a Tukey's multiple comparisons test tests between genotypes as well as per time point.