One-pot multi-substrate screening of ligation reactions using PNA tags

Aki Kohyama; Sofía Barluenga; Nicolas Winssinger

doi:10.1039/d5sc08732e

January 1, 2026Chemical ScienceOpen Access

One-pot multi-substrate screening of ligation reactions using PNA tags

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Key Points

To develop a platform for rapid discovery of chemical ligation reactions through simplified screening.
Developed a one-pot multi-substrate screening (OPMSS) platform.
Utilized peptide nucleic acid (PNA) tagging to facilitate the screening.
Combined various substrates in a single reaction environment.
Successfully identified multiple ligation reactions in a single experiment.
Demonstrated efficiency in constructing complex biomolecular architectures.

Implication

Demonstrates a novel platform for accelerating reaction discovery in biomolecular architecture using PNA tags.

Abstract

Chemical ligation is an essential tool for constructing complex biomolecular architectures. To accelerate reaction discovery, a one-pot multi-substrate screening (OPMSS) platform was developed, combining peptide nucleic acid (PNA) tagging with...

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Cite This Study

Kohyama et al. (2026) studied this question.

synapsesocial.com/papers/699a9d27482488d673cd2e60 https://doi.org/10.1039/d5sc08732e

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One-pot multi-substrate screening of ligation reactions using PNA tags

Key Points

Implication

Abstract

Discussion

Cite This Study

One-pot multi-substrate screening of ligation reactions using PNA tags

Key Points

Implication

Abstract

Discussion

Cite This Study

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