The research aims to develop a method for DNA transfection without vectors, enhancing genetic engineering techniques.
Utilized mechanoporation to deliver plasmid DNA directly into cells.
Targeted genetic modification to assess efficiency and safety.
Compared results to traditional vector-based delivery methods.
Demonstrated successful DNA delivery into target cells without adverse effects.
Showed improved efficiency over conventional methods.
Indicated potential applications in treating various diseases.
Abstract
Genetic engineering of cells has a range of applications in treating incurable diseases. Plasmid DNA is a popular choice of nucleic acid for cell engineering due to its low cost...