What question did this study set out to answer?

The aim is to reengineer enzymes to enhance their activity in ionic liquids for biocatalysis applications.

February 25, 2026Green Chemistry1 citationsOpen Access

Lessons learned on how to reengineer enzymes for improved performance in ionic liquids: insights from BSLA saturation mutagenesis library

SMShuaiqi MengNanjing Normal University YJYu JiBeijing University of Chemical Technology MYMaxine YewTianjin University of Traditional Chinese Medicine

Key Points

The aim is to reengineer enzymes to enhance their activity in ionic liquids for biocatalysis applications.
Utilized a saturation mutagenesis library to introduce variations in enzyme structure.
Conducted assays to test enzyme performance in different ionic liquid environments.
Evaluated the effects of structural changes on enzyme activity and stability.
Identified specific mutations that significantly enhance enzyme performance in ionic liquids.
Demonstrated improved substrate solubilization and reaction efficiency.
Showed potential for environmentally friendly biocatalytic processes using reengineered enzymes.

Abstract

Ionic liquids (ILs) are attractive reaction media in biocatalysis due to their excellent substrate solubilization properties and their promotion of mild and often environmentally friendly reaction conditions. However, enzyme activity...

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