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February 25, 2026Wellcome Open Research0 citationsOpen Access

The chromosomal genome sequence of the lettuce sea slug, Elysia crispata (Mörch, 1863) (Plakobranchidae) and its associated microbial metagenome sequences

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MMMichael L. MiddlebrooksJLJose Victor LopezNCNicholas Curtis

Key Points

  • This research aims to provide a comprehensive genome assembly of the lettuce sea slug and analyze its associated microbial metagenome.
  • Sequenced the genomic DNA of Elysia crispata
  • Constructed a genome assembly with 15 chromosomal pseudomolecules
  • Annotated over 18,000 protein-coding genes using Ensembl
  • Sequenced chloroplast genomes of two algae found in the slug cells
  • Recovered six metagenome bins, including four high-quality MAGs.
  • The genome assembly is 542.56 megabases long with 87.52% scaffolded into pseudomolecules.
  • Identification of 18,436 protein-coding genes adds to functional understanding.
  • Chloroplasts from dietary algae remain active within slug cells.
  • Recovered microbial data includes high-quality MAGs, indicating diverse microbial interactions.

Abstract

We present a genome assembly from from a specimen of the clarki ecotype of an individual specimen of the lettuce sea slug Elysia crispata (lettuce sea slug; Mollusca; Gastropoda; Plakobranchidae). The genome sequence has a total length of 542.56 megabases. Most of the assembly (87.52%) is scaffolded into 15 chromosomal pseudomolecules. The mitochondrial genome has also been assembled, with a length of 14.17 kilobases. Additionally, we sequenced the chloroplast genomes of two dietary algae, Penicillus capitatus and P. lamourouxii , from plastids sequestered inside of slug cells where they remain photosynthetically active. Gene annotation of this assembly by Ensembl identified 18 436 protein-coding genes. From the metagenome data, we recovered six bins, of which four were high-quality MAGs.

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Cite This Study

Middlebrooks et al. (2026) studied this question.

synapsesocial.com/papers/699e91c4f5123be5ed04f781https://doi.org/10.12688/wellcomeopenres.26088.1
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