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To evaluate the effectiveness of a new method for sampling environmental DNA in sediment for community-based monitoring.

February 26, 2026PLoS ONEOpen Access

Accessible and cost-effective deployment of environmental DNA (eDNA) samplers for sediment conducive to supporting community-based surveys

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To evaluate the effectiveness of a new method for sampling environmental DNA in sediment for community-based monitoring.
Developed the FloppE-Dip method for passive sampling of sediment.
Compared FloppE-Dip with conventional filtration in detecting species’ DNA via real-time quantitative PCR.
Conducted tests on American bullfrog tissue and field surveys at coastal beaches.
FloppE-Dip detected Pacific sand lance eDNA at 14 out of 20 sites.
Copies per liter estimates from FloppE-Dip were 5-10 times lower than conventional filtration.
FloppE-Dip proved to be faster and cheaper than traditional filtration methods.

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Laboratory comparison shows passive sampling for eDNA is non-invasive and cost-efficient for monitoring biodiversity.

Dema et al. (2026) studied this question.

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