Pleiotropic Effects of Knockout Mutations in the and Genes Encoding Trehalose Biosynthesis Enzymes of

The and genes encoding trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase play an important role in protecting yeast and fungal cells from temperature stress, dehydration and oxidants, since resistance to these stresses requires accumulation of trehalose. In this work, it was shown that strains with knockout mutations in the and genes have greater resistance to heavy metal ions and alkaline stress compared to the parental strain. These mutations do not lead to significant changes in the polyphosphate content in the cells, indicating the absence of a direct relationship between phosphorus homeostasis and the ability to accumulate trehalose. In the cells of Δtps2 the change in localization of cell wall protein Bgl2p was observed: this protein appeared to be intracellular. It is assumed that the resistance of knockout strains to heavy metal ions and alkali is associated with additional pleiotropic effects of these mutations.