What question did this study set out to answer?

To develop a fluorescent sensor for detecting APE1 activity, crucial for breast cancer diagnosis.

February 28, 2026Analytical Methods0 citations

An "off-on" fluorescent sensor based on FRET and magnetic beads for APE1 activity detection in breast cancer cell lysates

ZRZhuozhuo Ren QZQiongdan Zhang YWYuxing WangHunan University of Traditional Chinese Medicine

Key Points

To develop a fluorescent sensor for detecting APE1 activity, crucial for breast cancer diagnosis.
Designed a fluorescent nanosensor using FRET technology.
Incorporated magnetic beads to enhance detection sensitivity.
Tested the sensor in breast cancer cell lysates.
Successfully detected APE1 activity with high sensitivity.
Demonstrated the 'off-on' mechanism of the fluorescent sensor.
Indicated potential applications for breast cancer biomarker detection.

Abstract

The activity of apurinic/apyrimidinic endonuclease 1 (APE1) is a key biomarker in breast cancer. We report an "off-on" fluorescent nanosensor (OOFN) that combines fluorescence resonance energy transfer (FRET) with magnetic...

AI से पूछें

Bookmark

View Full Paper