An "off-on" fluorescent sensor based on FRET and magnetic beads for APE1 activity detection in breast cancer cell lysates

Zhuozhuo Ren; Qiongdan Zhang; Yuxing Wang; Zhuoer Chen; Ziyi Zhou; Da Qian

doi:10.1039/d6ay00068a

January 1, 2026Analytical Methods

An "off-on" fluorescent sensor based on FRET and magnetic beads for APE1 activity detection in breast cancer cell lysates

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Key Points

To develop a fluorescent sensor for detecting APE1 activity, crucial for breast cancer diagnosis.
Designed a fluorescent nanosensor using FRET technology.
Incorporated magnetic beads to enhance detection sensitivity.
Tested the sensor in breast cancer cell lysates.
Successfully detected APE1 activity with high sensitivity.
Demonstrated the 'off-on' mechanism of the fluorescent sensor.
Indicated potential applications for breast cancer biomarker detection.

Implication

Demonstrates a fluorescent sensor detects APE1 activity in breast cancer, suggesting a novel biomarker for diagnosis.

Abstract

The activity of apurinic/apyrimidinic endonuclease 1 (APE1) is a key biomarker in breast cancer. We report an "off-on" fluorescent nanosensor (OOFN) that combines fluorescence resonance energy transfer (FRET) with magnetic...

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Cite This Study

Ren et al. (2026) studied this question.

synapsesocial.com/papers/69a286720a974eb0d3c01684 https://doi.org/10.1039/d6ay00068a

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An "off-on" fluorescent sensor based on FRET and magnetic beads for APE1 activity detection in breast cancer cell lysates

Key Points

Implication

Abstract

Discussion

Cite This Study

An "off-on" fluorescent sensor based on FRET and magnetic beads for APE1 activity detection in breast cancer cell lysates

Key Points

Implication

Abstract

Discussion

Cite This Study

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