What question did this study set out to answer?

This research aims to characterize purinergic signaling's role in hepatocellular carcinoma and how the tumor microenvironment affects treatment responses.

February 28, 2026MetabolitesOpen Access

Integrating Transcriptomics and 3D Spheroid Models Reveals Microenvironment-Dependent Purinergic Modulation in Hepatocellular Carcinoma

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Authors

ASArieli Cruz de Sousa AWAugusto Ferreira Weber VKVinícius Freitas Klain

Discussion

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Overview

This research demonstrates how purinergic modulation impacts treatment response in hepatocellular carcinoma, indicating important therapeutic implications.

Key Points

This research aims to characterize purinergic signaling's role in hepatocellular carcinoma and how the tumor microenvironment affects treatment responses.
Analyzed RNA-seq data from TCGA-LIHC to identify differentially expressed genes and prognostic markers.
Utilized 3D spheroid models of HepG2 cells to study the effects of sorafenib and doxazosin.
Conducted qPCR and flow cytometry to assess gene and protein expression changes.
High expression of ADA, NT5E, and ADORA1 is associated with poor overall survival in HCC.
Combination therapy significantly downregulated NT5E and ADORA1 expression in 3D models.
Enhanced apoptosis was observed in spheroids when treated with the combination therapy compared to monotherapy.

Cite This Study

Sousa et al. (2026) studied this question.

synapsesocial.com/papers/69a2877b0a974eb0d3c032ec https://doi.org/10.3390/metabo16030152

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February 28, 2026MetabolitesOpen Access

Integrating Transcriptomics and 3D Spheroid Models Reveals Microenvironment-Dependent Purinergic Modulation in Hepatocellular Carcinoma

View Full Paper

Ask AI

Bookmark

Authors

ASArieli Cruz de Sousa AWAugusto Ferreira Weber VKVinícius Freitas Klain

Discussion

Member takes

Overview

This research demonstrates how purinergic modulation impacts treatment response in hepatocellular carcinoma, indicating important therapeutic implications.

Key Points

This research aims to characterize purinergic signaling's role in hepatocellular carcinoma and how the tumor microenvironment affects treatment responses.
Analyzed RNA-seq data from TCGA-LIHC to identify differentially expressed genes and prognostic markers.
Utilized 3D spheroid models of HepG2 cells to study the effects of sorafenib and doxazosin.
Conducted qPCR and flow cytometry to assess gene and protein expression changes.
High expression of ADA, NT5E, and ADORA1 is associated with poor overall survival in HCC.
Combination therapy significantly downregulated NT5E and ADORA1 expression in 3D models.
Enhanced apoptosis was observed in spheroids when treated with the combination therapy compared to monotherapy.

Cite This Study

Sousa et al. (2026) studied this question.

synapsesocial.com/papers/69a2877b0a974eb0d3c032ec https://doi.org/10.3390/metabo16030152

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