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The research aims to assess how different doses of 3-NOP affect methane emissions and various performance metrics in Nellore cattle.

March 2, 2026

Effect of two-doses of 3-Nitrooxypropanol (3-NOP) on methane emissions, performance, rumen microbiome, and metabolomics in Nellore cattle.

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Trial evaluates the impact of 3-NOP on methane emissions in cattle, suggesting benefits for livestock management.

The research aims to assess how different doses of 3-NOP affect methane emissions and various performance metrics in Nellore cattle.
Conducted a completely randomized study with three treatment groups of Nellore bulls.
Administered either control diet or diets supplemented with 3-NOP at two different doses (65 mg and 85 mg 3-NOP/kg of dry matter).
Measured enteric methane emissions using the sulfur hexafluoride tracer gas technique over a 115-day trial.
Methane emissions reduced by 13.2% (3-NOP65) and 26.7% (3-NOP85).
No negative effects observed on final body weight or average daily gain (P > 0.89).
Dry matter intake increased linearly with 3-NOP inclusion (P = 0.05).
Rumen microbiome showed significant changes in the Euryarchaeota phylum (P = 0.01).
Metabolomics revealed key amino acids (proline, arginine, threonine) as discriminant metabolites in the 3-NOP85 group.

Amancio et al. (2026) studied this question.

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