The development of new composite wound dressing films that can ensure a moist environment while preventing bacterial growth led this research to obtain novel ternary biopolymer films as a carrier for amygdalin. Due to their accessibility, biocompatibility, and versatility, κ–carrageenan, hydroxypropyl methylcellulose, and gelatin were selected as matrix components. This novel film composite was characterized by Fourier–transform Infrared (FTIR) spectroscopy, Thermogravimetric (TG) and Derivative Thermogravimetric (DTG) analysis, and was evaluated in vitro against E. coli and S. aureus. Thermogravimetric analysis showed that increasing the amygdalin content gradually shifted the Tonset and Tmax values to higher temperatures, suggesting an improvement in the thermal stability of the composite matrix. In vitro results indicate that increasing the amygdalin concentration resulted in a bacteriostatic efficiency of up to 54% against E. coli, while exhibiting a plateau effect in bactericidal activity. In contrast, no bactericidal activity was observed against S. aureus cultures.
Okolišan et al. (2026) studied this question.