What question did this study set out to answer?

The study aims to examine how TGF-β1 influences O-glycosylation and induces an epithelial-mesenchymal transition in breast cancer cells.

Transforming Growth Factor-β Drives Epithelial Mesenchymal Transition and Reduces Synthesis of Unelaborated O-GalNAc Glycans in Breast Cancer Cells

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Experimental study demonstrates TGF-β1 alters O-glycosylation and induces EMT in breast cancer cells, indicating potential impact on metastasis.

The study aims to examine how TGF-β1 influences O-glycosylation and induces an epithelial-mesenchymal transition in breast cancer cells.
MCF-7 and T47-D breast cancer cells treated with TGF-β1 for up to 7 days.
Morphological changes assessed by confocal microscopy.
Expression of EMT markers evaluated using immunofluorescence and western blot.
Effect on O-glycosylation analyzed using Helix pomatia agglutinin (HPA) labelling.
TGF-β1 treatment caused morphological changes, leading to a mesenchymal-like phenotype.
Reduced epithelial marker E-cadherin levels were observed.
No significant induction of mesenchymal markers N-cadherin or vimentin was detected.
TGF-β1 significantly decreased synthesis of unelaborated Tn antigen.

Cull et al. (2026) studied this question.

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Experimental study demonstrates TGF-β1 alters O-glycosylation and induces EMT in breast cancer cells, indicating potential impact on metastasis.