Structure-guided engineering and molecular insights into the regioselectivity of a novel Fe(II)/2-ketoglutarate-dependent dioxygenase HoIDO for synthesis of (2S,3R,4S)-4-hydroxyisoleucine | Synapse
March 3, 2026
Structure-guided engineering and molecular insights into the regioselectivity of a novel Fe(II)/2-ketoglutarate-dependent dioxygenase HoIDO for synthesis of (2S,3R,4S)-4-hydroxyisoleucine
Key Points
The enzyme HoIDO demonstrates high regioselectivity for converting substrates into (2S,3R,4S)-4-hydroxyisoleucine, enhancing synthetic routes.
Identification of key structural features underscores the precision of this dioxygenase in its reactions, leading to improved outcomes.
Analysis using structure-guided engineering techniques reveals significant molecular insights into the enzyme’s behavior and selectivity.
These findings may enable optimized production strategies for pharmaceutical compounds relying on hydroxyisoleucine, though further investigation is warranted.