Synergistic TdT/CbAgo mediated protein-nucleic acid conversion for trace detection of neuron-derived exosomal Aβ42

Key Points

• Neuron-derived exosomal Aβ42 detection showed significant improvement with TdT and CbAgo synergy, enhancing diagnostic capabilities.
• A notable detection limit of 100 fM was achieved, demonstrating the sensitivity of the combined method for trace analysis.
• The approach utilized a protein-nucleic acid conversion technique integrating TdT and CbAgo in exosomal samples.
• This method highlights the potential for early diagnosis of neurodegenerative diseases through improved biomarker detection.