Determination of 1,3-Dihydroxyacetone in honey using 2,4-dinitrophenylhydrazine derivatization coupled with HPLC and its application in detecting adulteration

Key Points

• The method reveals 1,3-dihydroxyacetone as a key marker for detecting honey adulteration.
• Analysis using high-performance liquid chromatography (HPLC) effectively identifies this compound.
• The study employs derivatization with 2,4-dinitrophenylhydrazine for accurate detection.
• Findings highlight the importance of monitoring honey quality to prevent fraud.