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March 4, 20261 citationsOpen Access

Phage RNA Polymerases Minimize Double-Stranded RNA Formation During Transcription

Identification of Phage RNA Polymerases That Minimize Double-Stranded RNA By-Product Formation and Their Characterization via In Vitro Transcription

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Authors

LGLilian GöldelCBCarsten BornhövdJKJohannes Kabisch

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Overview

Genome-mining identifies RNA polymerases that reduce double-stranded RNA in therapeutics, suggesting enhanced stability.

Key Points

  • The research aims to identify RNA polymerases with minimal double-stranded RNA generation for enhanced therapeutic applications.
  • Genome mining approach to identify single-subunit RNA polymerases
  • Screening of a large meta database with selection of 74 sequences
  • Fluorescent RNA aptamer screening to detect promoters
  • Characterization of transcription conditions to reduce dsRNA generation
  • Two RNA polymerases identified with minimal dsRNA generation (0.001% and 0.02%)
  • Significant reduction of dsRNA contamination supports enhanced mRNA stability
  • Potential application in producing long RNAs like self-amplifying RNA

Cite This Study

Göldel et al. (2026) studied this question.

synapsesocial.com/papers/69a7cd7ed48f933b5eed9e84https://doi.org/10.3390/microorganisms14030564
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