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This research aims to understand how different sugars and microbial interactions affect biohydrogen production in dark fermentation.

March 5, 2026Biomass and BioenergyOpen Access

Lactic acid bacteria in dark fermentation: From competitor to syntrophic partner for biohydrogen production from complex sugars

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This research aims to understand how different sugars and microbial interactions affect biohydrogen production in dark fermentation.
Conducted batch fermentation experiments using mesophilic conditions with varied sugar types and concentrations.
Evaluated hydrogen yields from hexoses and pentoses under different conditions.
Assessed microbial network dynamics through 3D-EEM analysis of fermentation broth.
Hexoses-based substrates produced significantly more hydrogen compared to pentoses, with up to 102.09% higher yields.
Hydrogen production increased 2.26 times at high lactate concentrations (972.47 mg/L).
Lactic acid bacteria demonstrated specific partnerships with hydrogen-producing organisms, enhancing overall production efficiency.

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Batch experiments evaluate biohydrogen production efficiency from sugars, suggesting strategic microbial collaborations for improvement.

Zhao et al. (2026) studied this question.

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