Nicotiana benthamiana is widely used as a model plant for studying plant-pathogen interactions owing to its genetic background and experimental accessibility. Its allopolyploid genome, high susceptibility to diverse pathogens, and distinct composition of RNA silencing-related genes provide a suitable system for investigating host responses during infection. Studies in N.benthamiana have enabled detailed analyses of interactions with viruses, viroids, bacteria, fungi, and oomycetes, and have contributed to the characterization of antiviral RNA silencing, pattern-triggered immunity (PTI), effector-triggered immunity (ETI), and MAPK-mediated signaling pathways. Premature transcriptional termination resulting in a nonfunctional NbRDR1 constitutes a key factor influencing antiviral RNA silencing regulation and host–virus interactions in this species. N.benthamiana has also been used to dissect immune receptor-mediated signaling and downstream defense responses to non-viral pathogens, including reactive oxygen species production and programmed cell death. In addition, the availability of virus-induced gene silencing and Agrobacterium -mediated transient expression systems has facilitated functional analyses and comparative studies across plant species. This review integrates molecular insights from diverse pathosystems and evaluates limitations relevant to interpreting results obtained in N.benthamiana .
Yang et al. (Tue,) studied this question.