Dual-frequency ultrasonic-assisted extraction of pumpkin polysaccharides: Process optimization, structural characterization, and antioxidant activity

Yuyuan Duan; Wei Li; Yixiao Zhu; Junkai Chen; Jianfei Xue; Huizhu Wang; Shuai Chen

doi:10.1016/j.lwt.2026.119220

March 1, 2026LWTOpen Access

Dual-frequency ultrasonic-assisted extraction of pumpkin polysaccharides: Process optimization, structural characterization, and antioxidant activity

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Key Points

The aim was to optimize the extraction of pumpkin polysaccharides using dual-frequency ultrasound while characterizing their structure and antioxidant activity.
Applied dual-frequency ultrasound-assisted extraction (DF-UAE) at 20/40 kHz.
Compared extraction efficiency with hot-water extraction (HWE).
Utilized response surface optimization for extraction yield.
Conducted structural analysis through methylation and NMR analyses.
Evaluated antioxidant activity using ABTS•+ and •OH assays.
DF-UAE achieved a 1.78-fold higher extraction yield compared to HWE.
Extraction time reduced by 78% and specific energy consumption decreased by 90%.
DF-UAE-derived polysaccharides showed enhanced uronic acid content and pronounced antioxidant activities.
Identified PPs2 as a branched rhamnogalacturonan-I-type pectic heteropolysaccharide with specific linkages.

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Implication

The study demonstrates enhanced extraction efficiency and antioxidant properties of pumpkin polysaccharides using dual-frequency ultrasound in a sustainable manner.

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Cite This Study

Duan et al. (2026) studied this question.

synapsesocial.com/papers/69aa6eb1531e4c4a9ff58edc https://doi.org/10.1016/j.lwt.2026.119220

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Dual-frequency ultrasonic-assisted extraction of pumpkin polysaccharides: Process optimization, structural characterization, and antioxidant activity

Key Points

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Discussion

Cite This Study

Dual-frequency ultrasonic-assisted extraction of pumpkin polysaccharides: Process optimization, structural characterization, and antioxidant activity

Key Points

No abstract yet

Implication

Discussion

Cite This Study

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