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March 6, 2026Biosensors and BioelectronicsOpen Access

High affinity and selective DNA aptamers for the detection of tobramycin in eye drops

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Authors

XZXiaohan ZhangECEmily Hoi Pui ChaoQLQiushi Liu

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Overview

This research demonstrates high-affinity binding to tobramycin using DNA aptamers, suggesting improved detection in eye drops.

Key Points

  • The study aims to identify high-affinity DNA aptamers for accurate tobramycin detection.
  • Utilized a library-immobilization based SELEX strategy to select aptamers.
  • Determined binding affinity using isothermal titration calorimetry.
  • Characterized aptamer structure through mutation analysis, NMR spectroscopy, and circular dichroism.
  • Developed a label-free fluorescent biosensor using the selected aptamer.
  • The lead aptamer TOB-2 binds to tobramycin with a dissociation constant of 420 nM.
  • TOB-2 exhibited over 1000-fold weaker affinity for kanamycin.
  • The biosensor achieved a limit of detection of 29 nM for tobramycin in eye drops.

Cite This Study

Zhang et al. (2026) studied this question.

synapsesocial.com/papers/69aa6f3c531e4c4a9ff59537https://doi.org/10.1016/j.bios.2026.118569
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