What question did this study set out to answer?

To develop a simple and sensitive method for detecting telomerase activity.

March 6, 2026RSC Advances0 citationsOpen Access

Nanoparticle-based colorimetric biosensor for the detection of telomerase activity based on the peroxidase-mimicking activity of nanogold

LULe Thi Hoang UyenVietnam National University Ho Chi Minh City VDVo Thi Cam DuyenVietnam National University Ho Chi Minh City PTPhuoc Long TruongVietnam National University Ho Chi Minh City

Key Points

To develop a simple and sensitive method for detecting telomerase activity.
Developed a colorimetric assay using gold nanoparticles (AuNPs) for detection.
Utilized telomerase self-extension and exonuclease III cleavage for signal amplification.
Exploited the peroxidase-like activity of AuNPs to generate a color change for detection.
Achieved a detection limit of approximately 0.3 MCF-7 cells.
Demonstrated amplification-free detection without complex instrumentation.
Showed high sensitivity and low cost compared to traditional methods.

Abstract

A simple, low-cost, amplification-free and ultrasensitive assay was developed to detect telomerase via telomerase self-extension, exonuclease III cleavage, and peroxidase-like activity of AuNPs with detection limit of ∼0.3 MCF-7 cell.

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