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This study aims to explore the impact of glucagon on the development of the tibiotarsus in chick embryos.

March 6, 2026Open Access

A study of the histogenesis of the developing tibiotarsus in glucagon-injected chick embryos

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Histological analysis reveals glucagon's effects on calcification in chick embryos, suggesting differing influences from insulin.

This study aims to explore the impact of glucagon on the development of the tibiotarsus in chick embryos.
Chick embryos were injected with glucagon on days 8 and 10 of development.
Histological samples of tibiotarsi were collected on days 9, 11, and 12.
Histochemical tests measured acid phosphatase, acid mucopolysaccharides, and calcium levels.
No abnormalities in chondrification and ossification patterns were found due to glucagon.
Acid phosphatase and acid mucopolysaccharide staining showed no changes in glucagon-treated tibiotarsi.
Smaller glucagon concentrations delayed calcification, while larger concentrations initially delayed but later increased calcification.

David Paul Strum (1976) studied this question.

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