Generation of a human induced pluripotent stem cell line (FLENIi004-A) from an Autosomal-Dominant Alzheimer’s disease PSEN1 p.M146L patient

M. Vautier; Patricio Chrem Mendez; Guillermo Jerez Ferreyra; Victoria Massazza; Manuela Apecetche; María S. Rodriguez Varela; Giulia Clas; Micaela Nievas; Ricardo Allegri; Gustavo Sevlever; María Élida Scassa; Ezequiel Surace; Mariela C. Marazita; Leonardo Romorini

doi:10.1016/j.scr.2026.103958

March 1, 2026Stem Cell ResearchOpen Access

Generation of a human induced pluripotent stem cell line (FLENIi004-A) from an Autosomal-Dominant Alzheimer’s disease PSEN1 p.M146L patient

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Key Points

The research aimed to create a human induced pluripotent stem cell line that retains a specific genetic mutation associated with Alzheimer's disease.
Generated FLENIi004-A from peripheral blood mononuclear cells using a lentiviral vector.
Validated the presence of the PSEN1 p.M146L mutation in the stem cell line.
Confirmed pluripotency and ability to differentiate into three germ layers.
The FLENIi004-A hiPSC line maintained the PSEN1 p.M146L mutation.
The cell line demonstrated pluripotency capabilities.
Successful trilineage differentiation potential was established.

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Implication

Researchers generated a human stem cell line from an Alzheimer's disease patient, highlighting potential for studies in genetics.

Abstract

Human induced pluripotent stem cell (hiPSC) line FLENIi004-A was generated from peripheral blood mononuclear cells (PBMCs) using the lentiviral-hSTEMCCA-loxP vector. FLENIi004-A was derived from a 41-year-old Argentinean Caucasian male patient with Autosomal-Dominant Alzheimer’s Disease, who carried the heterozygous PSEN1 p.M146L mutation. The hiPSC line retained the PSEN1 mutation, and its pluripotency and trilineage differentiation potential were confirmed.

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Vautier et al. (2026) studied this question.

synapsesocial.com/papers/69af949670916d39fea4b961 https://doi.org/10.1016/j.scr.2026.103958

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Generation of a human induced pluripotent stem cell line (FLENIi004-A) from an Autosomal-Dominant Alzheimer’s disease PSEN1 p.M146L patient

Key Points

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Implication

Abstract

Discussion

Cite This Study

Generation of a human induced pluripotent stem cell line (FLENIi004-A) from an Autosomal-Dominant Alzheimer’s disease PSEN1 p.M146L patient

Key Points

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Implication

Abstract

Discussion

Cite This Study

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