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Evaluation reveals PCR and gene sequencing is essential for identifying challenging NTM strains, indicating a need for improved methods.
The limitations of MALDI-TOF MS in verifying difficult-to-classify NTM strains have been demonstrated. The findings emphasize that PCR-based molecular detection combined with gene sequence analysis remains the most reliable methodological approach for accurately identifying challenging NTM species in clinical practice.
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Long et al. (2026) studied this question.
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