What does this research mean for the field?

A single liquid chromatographic method effectively quantifies lutein and zeaxanthin across various matrices, including food and Caco-2 cells, with high precision and transferability. Novelty: ClaimNovelty.METHODOLOGICAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

The aim is to develop a reliable method for quantifying lutein and zeaxanthin in plants and biological samples.

March 13, 2026FoodsOpen Access

Quantification of Macular Carotenoids over a Wide Dynamic Range in Plant Matrices and Caco-2 Cells Using a Single Transferable Analytical Method

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The aim is to develop a reliable method for quantifying lutein and zeaxanthin in plants and biological samples.
Utilized alkaline hydrolysis extraction for effective compound retrieval.
Applied a single liquid chromatographic technique for separation and quantification.
Created a method compatible with both PDA and MS detection.
Tested across various matrices such as food, digesta, and Caco-2 cells.
Achieved excellent recoveries in spiked samples.
Showed acceptable relative standard deviations for validation parameters.
Proved potential for high-throughput analysis across diverse matrices.

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Implication

Quantifies macular carotenoids in various matrices, indicating a unified analytical method for enhanced measurement.

Cite This Study

Sutharsan et al. (2026) studied this question.

synapsesocial.com/papers/69b3abc502a1e69014cccf38 https://doi.org/10.3390/foods15060981

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Quantification of Macular Carotenoids over a Wide Dynamic Range in Plant Matrices and Caco-2 Cells Using a Single Transferable Analytical Method

Key Points

Discussion

Implication

Cite This Study

Quantification of Macular Carotenoids over a Wide Dynamic Range in Plant Matrices and Caco-2 Cells Using a Single Transferable Analytical Method

Key Points

Discussion

Implication

Cite This Study

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