A protocol demonstrates liver regeneration and fibrosis in zebrafish, indicating its relevance to human diseases.
Here, we present a protocol for studying liver regeneration in adult zebrafish using a hepatic cryoinjury technique. We detail the surgical procedure to induce localized necrosis using a cryoprobe. We then detail steps for liver harvesting and CUBIC-based tissue clearing for three-dimensional imaging. The protocol enables comparison of healthy and injured liver parenchyma within individual animals, recapitulating key features of human chronic liver disease, including fibrosis formation and resolution.
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Sande-Melón et al. (2026) studied this question.
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