Development of Direct Liquid–Liquid Extraction (LLE) of an Enzymatic Cascade En Route to Enlicitide Using an Automated, High-Throughput Screening Platform
Automated high-throughput screening identifies optimal conditions for isolating a macrocyclic peptide in biocatalysis, suggesting efficiency in purification processes.
Key Points
The research aims to develop an efficient method for isolating a macrocyclic peptide generated in an enzymatic cascade.
Developed a liquid-liquid extraction process for purification.
Employed an automated, vision-guided high-throughput screening platform.
Systematically evaluated hundreds of process conditions.
Achieved 93% recovery of the macrocyclic peptide product.
Obtained no detectable protein residue post-extraction.
Streamlined purification process for biocatalytic applications.