Efficient Synthesis of Pyruvic Acid from Biomass based on Gas‐Liquid‐Solid Triphase Bioelectrochemical Cascade Reaction

The enzymatic synthesis of pyruvic acid (PyA) from biomass‐derived lactic acid (LA) using oxidases under mild conditions represents a promising sustainable route. However, its efficiency is hindered by insufficient O 2 mass transfer and undesired PyA degradation resulting from the enzymatic byproduct H 2 O 2 . Herein, we present an gas‐liquid‐solid triphase bioelectrochemical reaction system that can address these two challenges symmetrically. This design ensures an efficient supply of O 2 from the air phase to the lactate oxidase (LOx) active zone, thereby overcoming the mass‐transfer constraint of oxygen. Simultaneously, the in situ electro‐oxidation of H 2 O 2 minimizes the degradation of PyA. Consequently, the reaction system achieves a production rate of 54.34 µM h −1 U −1 , which is 63‐fold greater than that of conventional liquid‐solid diphase enzymatic systems. Mathematical modeling and electrochemical studies revealed that enhanced local O 2 levels and efficient H 2 O 2 removal are the keys to superior performance. This work provides a sustainable and efficient route for PyA biosynthesis while demonstrating the potential of engineered bioelectrocatalytic environments for green synthesis.