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March 14, 2026Journal of Translational MedicineOpen Access

The PI3K–AKT pathway mediates the imbalance of bone marrow macrophage polarization in patients with immune thrombocytopenia

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Authors

MSMeng-Zhu ShenPeking UniversityHZHong-Yan ZhaoPeking UniversityYYYa-Ting YuPeking University

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Implication

RNA sequencing reveals altered megakaryopoiesis in ITP, indicating PI3K–AKT pathway involvement in macrophage function.

Key Points

  • This research aims to investigate the role of the PI3K–AKT pathway in bone marrow macrophage polarization in immune thrombocytopenia.
  • Constructed bone marrow macrophage-specific PI3K-knockout mice using CRISPR/Cas9 technology.
  • Performed RNA sequencing on sorted megakaryocytes and evaluated their functions including platelet production and apoptosis.
  • Assessed macrophage polarization and cytokine levels through ELISA and evaluated migration and phagocytosis abilities.
  • Increased M1/M2 macrophage ratio and impaired megakaryopoiesis identified in PI3K-knockout mice compared to controls.
  • RNA-seq showed decreased levels of the platelet activation pathway in megakaryocytes.
  • Clinical samples from ITP patients showed increased M1/M2 ratio and downregulated p-PI3K and p-AKT in macrophages.

Cite This Study

Shen et al. (2026) studied this question.

synapsesocial.com/papers/69b4b9fb18185d8a398024d0https://doi.org/10.1186/s12967-026-07998-2
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