Study demonstrates effective detection of multidrug-resistant tuberculosis using Surface Plasmon Resonance biosensing techniques, indicating potential as a diagnostic tool.
Key Points
This research aims to evaluate the effectiveness of Surface Plasmon Resonance biosensing for detecting multidrug-resistant tuberculosis.
Used a Surface Plasmon Resonance biosensor chip for detection of genes related to MDR-TB.
Utilized single-stranded deoxyribonucleic acids (ssDNA) targets and thiolated probes.
Functionalization of probes to gold nanoparticles was confirmed with UV–vis and DLS analyses.
Detection of the selected genes showed distinct resonance angles for complementary vs. non-complementary targets.
Resonance angles for IS6110, InhA, KatG, and RpoB genes ranged from 45.53° to 46.91° depending on hybridization.
SPR biosensing demonstrated potential as a refined diagnostic method for MDR-TB.