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This study aims to understand the impact of manure on nitrogen cycling and soil multifunctionality during fertilizer substitution.

Partial substitution of chemical fertilizer with manure enhances soil multifunctionality and plant biomass through shifts in nitrogen forms and key nitrogen-associated microbial taxa

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This study aims to understand the impact of manure on nitrogen cycling and soil multifunctionality during fertilizer substitution.
Established four nitrogen-equivalent treatments involving chemical fertilizer and matured pig manure.
Analyzed shifts in nitrogen forms and microbial communities in response to fertilizer type.
Investigated relationship between specific bacterial taxa and nitrification/denitrification processes.
70% chemical fertilizer + 30% matured pig manure enhanced total nitrogen pool and soil multifunctionality.
Treatment reduced iron-manganese oxide form nitrogen while increasing bioavailable nitrogen forms.
Ammonia-oxidizing bacteria correlated with nitrification, while ammonia-oxidizing archaea showed sensitivity to manure addition.

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Experimental treatments demonstrated improvements in nitrogen cycling and microbial activity in soil, indicating effective manure use.

Wang et al. (2026) studied this question.

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