AMPK inhibition and elevated angiogenin are associated with tRNA fragmentation in the male germline exposed to a high-fat diet

The existence of an inherent rewiring of metabolic regulation has been demonstrated through studies of obese mice that are fed a normal diet and produce offspring with metabolic disorders. tRNA-derived fragments (tRFs) have been suggested as a potential mediator of this inheritance. To explore a mechanism underlying metabolic stress-induced tRNA fragmentation, we examined the effects of a HFD on tRFs in sperm. Small RNA sequencing on sperm revealed that HFD-induced metabolic changes affect the tRF profiles, showing a trend of decreased 5’-derived tRFs and increased 3’-derived tRFs, suggesting a shift in tRNA cleavage patterns under HFD feeding. In conjunction with the alteration of tRFs, the expression of Ang , which encodes the ribonuclease angiogenin, was significantly increased in the testis. Transcriptome and metabolome analyses indicated that AMPK-mTOR signaling pathway was the possible mediator of these effects, with decreased AMPK activity and increased mTOR activity confirmed at the protein level in the testis. Moreover, in vitro experiments showed that AMPK inhibition led to increased angiogenin expression level and alterations in tRF profile. In vitro angiogenin induction experiments, designed to mimic HFD conditions, produced changes in tRF profiles similar to those observed in HFD-fed mice, although the resulting tRF profiles did not completely recapitulate the in vivo HFD-induced profiles. Our findings suggest that HFD-induced metabolic stress inhibits AMPK during spermatogenesis, leading to increased Ang expression and altered tRF remodeling. • Metabolic stress from a high-fat diet alters sperm tRNA-derived fragment profiles. • HFD-induced metabolic stress shifts sperm tRF composition toward increased tRF3. • AMPK regulates angiogenin expression through mTOR-independent mechanisms. • Angiogenin induction under HFD conditions drives alteration of sperm tRF profiles.