What question did this study set out to answer?

To comprehensively map three-dimensional spatial interactions across the entire human genome and characterize the biophysical principles governing chromatin folding.

October 8, 2009ScienceOpen Access

Comprehensive Mapping of Long-Range Interactions Reveals Folding Principles of the Human Genome

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Authors

ELErez Lieberman-AidenBaylor College of Medicine NBNynke L. van BerkumUniversity of Massachusetts Chan Medical School LWLouise WilliamsNew England Biolabs (United States)

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Implication

Genome-wide mapping reveals three-dimensional chromatin folding principles in human cells, demonstrating dense knot-free packaging via a fractal globule conformation.

Key Points

To comprehensively map three-dimensional spatial interactions across the entire human genome and characterize the biophysical principles governing chromatin folding.
Developed Hi-C, an experimental method combining proximity-based ligation with massively parallel sequencing to identify spatial proximity between genomic loci.
Constructed whole-genome spatial contact maps of human cells at a 1-megabase resolution.
Confirmed the existence of chromosome territories and revealed that the human genome is organized into two distinct compartments separating open, active chromatin from closed, inactive chromatin.
Identified that chromatin at the megabase scale conforms to a knot-free fractal globule polymer structure rather than an equilibrium globule, enabling high packaging density with rapid folding and unfolding capability.

Cite This Study

Lieberman-Aiden et al. (2009) studied this question.

synapsesocial.com/papers/69b87bc77aaa581b6b02d363 https://doi.org/10.1126/science.1181369

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Implication

Genome-wide mapping reveals three-dimensional chromatin folding principles in human cells, demonstrating dense knot-free packaging via a fractal globule conformation.

Comprehensive Mapping of Long-Range Interactions Reveals Folding Principles of the Human Genome

Discussion

Member takes

Implication

Key Points

Cite This Study

Comprehensive Mapping of Long-Range Interactions Reveals Folding Principles of the Human Genome

Discussion

Member takes

Implication

Key Points

Cite This Study

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