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This research aims to develop an enzyme-free electrochemical sensor for glucose detection in biological fluids.

Enzyme‐Free Detection of Glucose in Blood and Saliva Using Silver Decorated Poly( o ‐phenylenediamine) Nanostructure

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An electrochemical sensor detects glucose in blood and saliva, indicating high accuracy and selectivity.

This research aims to develop an enzyme-free electrochemical sensor for glucose detection in biological fluids.
Developed a self-assembled monolayer of poly(o-phenylenediamine) on a glassy carbon electrode.
Decorated the layer with silver nanoparticles through thermal reduction.
Evaluated surface morphology using FESEM and EDAX elemental mapping.
Analyzed electrochemical properties via cyclic voltammetry in 0.1 M NaOH electrolyte.
Tested sensor's response to glucose and common interfering biomolecules.
Achieved a limit of detection of 5.5×10−7 mg dL−1.
Demonstrated excellent sensitivity of 4.4 µA µL−1 cm−2 in a linear range of glucose concentration.
Achieved an average accuracy of ~101% for glucose detection in raw human blood.
Confirmed glucose concentration measurement in human saliva.

Deo et al. (2026) studied this question.

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