Computational redesign and directed evolution of a lanthanide-dependent photoredox enzyme for enantioselective diol cleavage

Puntos clave

• The research aims to optimize engineered enzymes for efficient diol cleavage using lanthanide-dependent photoredox reactions.
• Developed PhotoLanZymes (PLZ) targeting specific radical mechanisms.
• Applied computational redesign strategies to enhance enzyme activity.
• Conducted directed evolution to refine enzyme properties.
• Achieved higher activity and selectivity in diol cleavage reactions.
• Demonstrated successful use of lanthanide ions in facilitating radical processes.
• Highlighted the potential for practical applications in synthetic chemistry.