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Developing a robust multiplex CRISPR/Cas12i3‐5M system for trait stacking in soybean | Synapse

March 19, 2026Journal of Integrative Plant Biology2 citations

Developing a robust multiplex CRISPR/Cas12i3‐5M system for trait stacking in soybean

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WLWenxin Lin HWH. J. Wu HKHuaqin Kuang

Key Points

This research aims to develop a high-efficiency multiplex gene editing system to improve soybean traits.
Utilized CRISPR-Cas12i3-5M technology for gene editing
Targeted 13 specific sites in the soybean genome
Generated germplasm with modified oil composition
Achieved high oleic acid content in soybean
Eliminated beany flavor in the modified soybeans
Demonstrated efficiency in multiplex editing across multiple targets

Abstract

The high-efficiency multiplex gene editing technology based CRISPR-Cas12i3-5M is capable of simultaneously editing 13 target sites in soybean, and was used to generate germplasm with high oleic acid content and no beany flavor.

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Cite This Study

Lin et al. (Mon,) studied this question.

synapsesocial.com/papers/69bb92be496e729e62980466 https://doi.org/https://doi.org/10.1111/jipb.70233