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March 19, 2026The Plant JournalOpen Access

Controlling GRF4 ‐ GIF1 expression for efficient, genotype‐independent transformation across wheat cultivars

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Authors

SHSadiye HaytaMSMark A. SmedleyMBMeltem Bayraktar

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Overview

Researchers enhance wheat transformation efficiency across cultivars, suggesting improvements in food security and breeding.

Key Points

  • The study aims to improve transformation efficiency in wheat using GRF4-GIF1 expression across various cultivars.
  • Developed an Agrobacterium-mediated transformation protocol.
  • Tested across multiple tetraploid and hexaploid wheat cultivars.
  • Adjusted selection pressure, zeatin concentration, and promoter choice to enhance transformation efficiency.
  • Implemented tissue-specific promoters and heat-inducible excision strategies to reduce negative effects.
  • Achieved consistent successful transformation across multiple wheat genotypes.
  • Improved transformation rates while minimizing reduced fertility and spikelet number.
  • Established a versatile platform for gene function studies and gene editing in wheat.

Cite This Study

Hayta et al. (2026) studied this question.

synapsesocial.com/papers/69bb9300496e729e62980cf0https://doi.org/10.1111/tpj.70799
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