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September 17, 2025Open Access

Choosing the Best Route: Comparative Optimization of Wheat Transformation Methods for Improving Yield by Targeting TaARE1-D with CRISPR/Cas9

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Authors

MTMümin Ibrahim TekKBKübra BUDAKPSPelin Sarıkaya

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Overview

This study demonstrates improved yield through optimized transformation in wheat using CRISPR/Cas9 targeting TaARE1-D.

Key Points

  • Transformation efficiency reached 66.84% for immature embryos, significantly enhancing yield potential.
  • Three methods were optimized, leading to a more than tenfold increase in transformation success compared to previous rates.
  • The approach involved genetic engineering via CRISPR/Cas9 to knockout TaARE1-D, promoting better nitrogen uptake efficiency.
  • These protocols may accelerate the application of gene editing in wheat, crucial for global food security.

Cite This Study

Tek et al. (2025) studied this question.

synapsesocial.com/papers/68d45e6a31b076d99fa5f0cahttps://doi.org/10.1101/2025.09.11.675438
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Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Choosing the best route: Comparative optimization of wheat transformation methods for improving yield by targeting TaARE1-D with CRISPR/Cas92026 · 1 citations
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  4. 4Controlling <scp>GRF4</scp> ‐ <scp>GIF1</scp> expression for efficient, genotype‐independent transformation across wheat cultivars2026 · 3 citations
  5. 5Rapid Genetic Stabilisation of Transgenic and Edited Wheat Through Transformation of Immature Haploid Embryos2025 · 2 citations