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March 21, 2026Microbial Cell FactoriesOpen Access

Scalable secretory production of influenza A (H1N1) hemagglutinin HA1 in Pichia pastoris through expression and process optimization

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Authors

ZGZeliang GuoHenan University of Science and TechnologyWDWenting DingHenan University of Science and TechnologyCYChendi YangHenan University of Science and Technology

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Implication

Demonstrates successful expression of influenza HA1 in Pichia pastoris, indicating a scalable method for viral antigen production.

Key Points

  • The aim is to optimize the production of influenza A hemagglutinin HA1 using Pichia pastoris as a microbial expression system.
  • Codon-optimized HA1 gene expression under AOX1 promoter
  • Selection of multicopy integrants using G418
  • Optimization of expression conditions in shake-flask culture
  • Purification via Ni-NTA affinity chromatography
  • Functional validation of antibodies in murine model
  • Recombinant HA1 protein produced at 0.375 g/L in culture supernatant
  • Purity of purified protein exceeded 95%
  • N-linked glycosylation confirmed through PNGase F digestion
  • Induction of rHA1-specific antibodies and hemagglutination-inhibiting activity observed

Cite This Study

Guo et al. (2026) studied this question.

synapsesocial.com/papers/69be34d16e48c4981c672e86https://doi.org/10.1186/s12934-026-02985-0
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