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March 21, 2026Journal of Clinical Microbiology3 citationsOpen Access

Genomic epidemiology of Enterococcus faecium bloodstream infections during a VanB-type VRE peak reveals an oligoclonal scenario: an observational study at a German university hospital (2017–2022)

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LKLeonard KnegendorfASAnna SommerCBClaas Baier

Key Points

  • The study aims to understand the epidemiology and transmission dynamics of vancomycin-resistant Enterococcus faecium in a hospital setting.
  • Documented shifting E. faecium populations and variants in bloodstream isolates
  • Conducted virulence gene profiling of infections
  • Used core genome multilocus sequence typing and split k-mer analysis
  • Integrated genomic analyses with epidemiological tracking
  • Predominance of hospital-associated variants across both resistant and susceptible strains
  • Identification of complex epidemiological dynamics including oligoclonal transmission
  • Differentiation between monoclonal outbreaks and broader transmission patterns

Abstract

Vancomycin-resistant Enterococcus faecium is an increasingly important nosocomial pathogen worldwide. Understanding its epidemiology and transmission dynamics is critical to effectively control its spread. This study documents shifting E. faecium populations within a high-risk hospital environment, including both vancomycin-resistant and vancomycin-susceptible bloodstream isolates. Virulence gene profiling demonstrated that hospital-associated variants predominated across all major lineages, independent of vancomycin resistance, indicating that hospital adaptation is a common feature of both resistant and susceptible populations. Detailed genomic analyses, combining core genome multilocus sequence typing with high-resolution split k-mer analysis, integrated with comprehensive epidemiological tracking, were critical to accurately depict complex epidemiological dynamics. This combined approach allows precise differentiation between monoclonal outbreaks and oligoclonal transmission, enabling more targeted infection prevention and control strategies.

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Cite This Study

Knegendorf et al. (2026) studied this question.

synapsesocial.com/papers/69be35f96e48c4981c6747c5https://doi.org/10.1128/jcm.01044-25
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