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The research aims to evaluate kidney quality using multimodal assessment during 24 hours of normothermic machine perfusion.

March 21, 2026Artificial OrgansOpen Access

Multimodal Assessment of Kidney Quality During 24‐h of Normothermic Machine Perfusion

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Demonstrates kidney viability assessment during machine perfusion, suggesting new biomarkers for transplantation.

The research aims to evaluate kidney quality using multimodal assessment during 24 hours of normothermic machine perfusion.
Thirteen porcine kidneys were perfused for 24 hours with an autologous leukocyte-filtered blood-based perfusate.
Functional, biochemical, and morphological assessments were conducted to evaluate kidney quality.
Microperfusion metrics were analyzed using near-infrared and tissue hemoglobin techniques.
Biomarker analysis focused on levels of KIM-1 and IGFBP-7 in proximal tubular cells.
Histological grading and real-time confocal assessment were performed to evaluate cellular architecture.
Stable hemodynamics and sustained urine output indicated preserved kidney viability.
Microperfusion improved significantly within two hours of perfusion.
Progressive injury in proximal tubular cells, with increased vacuolization and reduced microvilli density, was observed.
Overall OXPHOS capacity declined with perfusion duration, though ATP production efficiency remained stable.
Histological assessment showed no significant alterations over time.

Pühringer et al. (2026) studied this question.

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