ABSTRACT Hispidin, a secondary metabolite predominantly found in medicinal fungi has long been recognized for its broad pharmacological potential. This review aimed to comprehensively evaluate the pharmacological promise of hispidin by systematically synthesizing evidence from in vitro, in vivo, and in silico investigations. We focused on its assessed activities including antimicrobial effects, wound healing promotion, antioxidant properties, anti‐inflammatory actions, antiviral effects, and anticancer potential, while analyzing their underlying molecular mechanisms through interactions with specific targets. In vitro experiments demonstrated potent antioxidant and anti‐inflammatory activities, with hispidin modulating major pathways including Nrf2 and NF‐κB, and downregulating cytokines such as TNF‐α and IL‐6. Anticancer potential was evidenced through induction of apoptosis, cell cycle arrest, and inhibition of metastasis across multiple cancer cell lines. In conclusion, hispidin demonstrates potent antimicrobial activity against S. epidermidis via membrane disruption; accelerates wound healing through fibroblast migration and collagen deposition; exerts antioxidant effects by elevating SOD/GPx and reducing MDA; suppresses inflammation via NF‐κB inhibition; and shows anticancer promise through ROS‐induced apoptosis, a mechanism further supported by molecular docking studies predicting high‐affinity binding to some related kinases. These computational insights, which correlate with observed antiproliferative effects, collectively establish hispidin's therapeutic versatility across these domains.
Khedidja Benarous (Sun,) studied this question.