Evaluation of polymer interventions to reduce hemolysis in rodent liver NMP models, suggesting potential clinical applications.
Key Points
This research aims to assess the effectiveness of poloxamer 188 and Ficoll PM70 in reducing hemolysis during liver normothermic machine perfusion in rodent models.
Conducted machine perfusion using rat livers with RBC-based perfusates including P188 and/or Ficoll PM70 and an untreated control.
Measured hemolysis through free hemoglobin concentration using a NanoDrop spectrophotometer.
Analyzed hemodynamic stability, liver injury markers, and histological samples to assess graft viability.
P188 and Ficoll PM70 significantly reduced free hemoglobin levels in livers from donation after brain death models.
Combined use of P188 and Ficoll PM70 further reduced hemolysis in warm ischemia injury models and preserved liver sinusoidal endothelial cells.
The reduction in hemolysis did not improve hemodynamic parameters during perfusion.