The In Vitro Antioxidant, Antibacterial Activities and Cytotoxicity ofMercapto-1,2,4-triazole and their N-Arylacetamide Derivatives Basedon Iso- and Terphthalic Acids

introduction: Triazole compounds and their Acetamide derivatives have received great attention due to their intriguing pharmacological properties. They have become increasingly useful and important in constructing bioactive and functional molecules. materials and methods: Compounds were derived from isophthalic and terephthalic acids. The compounds 6a-d were prepared by reacting the hydrazides of acids with various aryl isothiocyanate followed by refluxing with potassium hydroxide for 6 hours. Different 2-bromo-N-(4-substitutedphenyl) acetamide 3a-f were prepared by reacting different substituted anilines with bromoacetyl bromide in an aqueous solution of sodium carbonate. The compounds 7a-p were then derived by reacting 6a-d with 3a-f for about 30 minutes in an ethanolic solution of potassium hydroxide. The new compounds were fully characterized by 1H-NMR, 13C-NMR, and IR spectroscopy. ABTS and DPPH assays were performed to explore the antioxidant and antimicrobial activity of the compounds 6a-c and 7a-p investigated by employing the broth micro broth dilution method. The cytotoxicity of the newly synthesized compounds was evaluated using CCK-8 kit. results: New compounds were produced with good yields of 60-93%. Thiol compounds 6a-c demonstrated radical inhibition behavior. The inhibition percentage increases with increasing concentration of the compounds in both scavenging assays. Most new compounds 6a-c and 7a-p displayed a MIC value of 128 µg/mL against examined gram negative and gram positive bacteria. All new compounds tested in this study were relatively safe in normal cells and did not instigate undesirable toxic effects. discussion: - conclusion: new compounds were successfully synthesized with good yield, fully characterized, and proved radical scavenging, moderate antibacterial activity and didn’t show desirable cytotoxicity in normal cells.