Rejuvenation of mesenchymal stromal cells via partial reprogramming enables scalable generation of transcriptionally diverse MSC libraries

Key Points

• To explore a rejuvenation strategy for mesenchymal stromal cells that enhances their diversity and scalability for therapeutic applications.
• Implementing a non-integrating rejuvenation strategy
• Defining operational characteristics of rejuvenated MSCs
• Generating diverse MSC libraries for testing
• Established a new definition for rejuvenated MSCs (rej-MSCs)
• Created a scalable platform for MSC manufacturing
• Demonstrated increased transcriptional diversity in MSCs