What question did this study set out to answer?

To evaluate the feasibility and reliability of liquid biopsy using cerebrospinal fluid (CSF) and plasma for glioma diagnosis.

Abstract A055: Glioblastoma liquid biopsy: Maximizing insights from DNA traces in cerebrospinal fluid and blood plasma

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Investigates liquid biopsy for glioma molecular diagnosis, indicating its potential and limitations in clinical settings.

To evaluate the feasibility and reliability of liquid biopsy using cerebrospinal fluid (CSF) and plasma for glioma diagnosis.
Examined glioma tissue samples from two cohorts (n=45 and n=39)
Collected peritumoral CSF and plasma or LP-CSF
Analyzed cell-free DNA using NGS, dPCR, and BEAMing PCR
Correlated clinical features with CSF DNA characteristics
Compared molecular diagnosis from LB with histopathological diagnosis.
Peritumoral CSF contained abundant high-molecular weight DNA, while LP-CSF had insufficient DNA for NGS
60% of cases achieved tissue-agnostic glioma classification using the ITEC protocol
Absence of tumor DNA in LP-CSF was linked to favorable prognosis
Plasma cfDNA analysis showed limited utility
GBM stem-like cells released DNA in EVs with over 50% detection of tumor mutations.

Boccaccio et al. (2026) studied this question.

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