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The research aims to enhance 1,5-pentanediol production in E. coli by identifying and manipulating key metabolic genes through transcriptomics.

March 25, 2026MicroorganismsOpen Access

Enhancing Synthesis Efficiency in Microbial 1,5-Pentanediol Production Through Transcriptomics-Informed Metabolic Engineering of Escherichia coli

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Comparative transcriptomics enhances 1,5-PDO biosynthesis in engineered E. coli, suggesting new genetic targets for optimization.

The research aims to enhance 1,5-pentanediol production in E. coli by identifying and manipulating key metabolic genes through transcriptomics.
Conducted comparative transcriptomic analysis between 1,5-PDO-producing and parental strains.
Identified 1384 significantly differentially expressed genes across growth phases.
Selected 20 candidate metabolic genes for functional validation using plasmid overexpression and CRISPR interference.
Integrated fecA and deleted gadA in optimized strain S7 for enhanced productivity.
Strain S7 produced 1.7 g/L of 1,5-PDO in shake flasks, a 13.3% increase over the parental strain.
Improved glucose yield to 0.18 mol/mol, reflecting a 50% increase.
In fed-batch fermentation, strain S7 achieved 12.45 g/L of 1,5-PDO and a glucose yield of 0.26 mol/mol.
Carbon conversion efficiency improved by 15.6% compared to the parental strain, alongside a 7.6% increase in biomass.

Deng et al. (2026) studied this question.

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